α-mannosidases

α(1-6) Core Mannosidase

α (1-6) Core Mannosidase (α-d-mannoside mannohydrolase, EC 3.2.1.24) cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the β-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides. The presence of fucose linked to the core N-acetylglucosamine has no effect on cleavage. The enzyme may inhibit other mannosidases if a noncleavable α(1-6) mannose is present on the substrate. It should therefore always be added subsequent to digestion by other mannosidases.

1 U/ml - 60 mU for 60 μL

GE60 datasheet pdf



α (1-2,3,6) Mannosidase

α (1-2,3,6) Mannosidase (α-D-mannoside mannohydrolase EC 3.2.1.24) cleaves all α(1-2,3,6)-linked mannose. The enzyme is prepared from Jack Bean. α(1-2,3,6) mannosidase is useful for determining mannose linkages.

10 U/ml - 600 mU for 60 μL

GE62 datasheet pdf