β (1-4) Galactosidase
β(1-4) Galactosidase (β-D-galactoside galactohydrolase, EC 3.2.1.23) releases only β(1-4)- linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. β(1-4) galactose is by far the most common linkage found in N-linked oligosaccharides. The enzyme is as active on tetraantennary oligosaccharides as on disaccharides containing β(1-4)-linked galactose. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose. Up to 100 υg of asialofetuin can be completely β(1-4)-degalactosylated in less than 1 hour using 3 mU of enzyme.
3 U/ml - 180 mU for 60 μL
GE12 datasheet pdf
β(1-3,6) Galactosidase
β(1-3, 6) Galactosidase (β-D-galactoside galactohydrolase, EC 3.2.1.23) cleaves β(1-3)- and β(1-6)-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. β(1-4)-linked galactose is cleaved at <1% of the β(1-3) rate, and only on linear oligosaccharides. Fucose, but not sialic acid, linked to the penultimate N-acetylglucosamine will block cleavage of the galactose
50 U/ml - 3 U for 60 μL
GE14 datasheet pdf
β(1-3,4,6) Galactosidase recombinant
β(1-3,4, 6) Galactosidase (β-D-galactoside galactohydrolase, EC 3.2.1.23) cleaves β(1-3),
β(1-4) and β(1-6)-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. . Fucose, but not sialic acid, linked to the penultimate N-acetylglucosamine will block cleavage of the galactose
6 U/ml - 360 mU for 60 μL
GE15 datasheet pdf
β(1-3, 4, 6) Galactosidase Bovine testes
Cleaves all β(1-3) and β(1-4) linked non-reducing, terminal galactose. β(1-6) linked galactose is released at a slower rate. The enzyme is a glycoprotein. Source: Bovine Testes. Must be stored frozen.
2.5 U/ml - 500 mU for 200 μL
GE13 datasheet pdf
