Enzymatic Glycoprotein Deglycosylation Kit
Kit includes the enzymes and buffers required to remove all N-linked oligosaccharides and most O-linked sugars from 2 mg of glycoprotein. The enzymes are packaged separately in individual 20 ul vials. This gives researchers the flexibility to characterize the glycans attached to their glycoprotein more fully than with the premixture of enzymes. Appropriate use of individual glycosidases allows the determination of sialyation (use of neuraminidase only) and the specific presence of N-linked glycans (using PNGase F only) and O-linked glycans (using neuraminidase, ß-galactosidase, Endo-O-Glycosidase, and ß-N-acetyglucosaminidase)
Includes:
PNGase F,
neuraminidase,
ß-Galactosidase,
ß-N-acetyglucosaminidase and
Endo-O-glycosidase.
This kit also includes buffer, denaturant solution, Triton X, and fetuin glycoprotein standard.
GE52 - Enzymatic Protein Deglycosylation Kit datasheet pdf
Enzymatic Glycoprotein Deglycosylation Premix Kit
Similar to GE52 but the 5 enzymes are premixed in one 20 ul vial. Fetuin control is not included.
For researcher who wish to remove all glycans without individual enzyme analysis.
GE50 Use GE52 data sheet for instructions
N-acetylneuraminic Acid Quantitation Kit
Contains all the reagents needed to quickly and accurately quantitate sialic acids, including N-acetylneuraminic acid (NANA) and N-glycolylneuraminic acid (NGNA). This kit includes reagent for 25 assays, each assay measuring from 1-200 nmoles of sialic acid.
GE57 datasheet pdf
Endoglycosidase F1, F2 and F3 Sampler Kit
Contains 20 ul each of Endo F1, F2 and F3 with two reaction buffers.
GE53 - see individual datasheets for specificity
